Generator
Regional

Part:BBa_K106676:Design

Designed by: Jacinto Chen   Group: iGEM08_UCSF   (2008-10-28)

Regional silencing construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1604
    Illegal PstI site found at 617
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1604
    Illegal PstI site found at 617
    Illegal NotI site found at 977
    Illegal NotI site found at 2339
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2322
    Illegal XhoI site found at 263
    Illegal XhoI site found at 1592
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1604
    Illegal PstI site found at 617
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1604
    Illegal PstI site found at 617
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2247


Design Notes

To test regional silencing: Cyc1P-mCherry-Adh1t-8xLexAOps-Cyc1P-GFP-Adh1t

Combination of Aar1 assembly (GFP cassette) and subclone (Kpn1-tailed mCherry cassette).

Source

none.

References